GB 31604.55-2023 (English Version)

Standard No.: GB 31604.55-2023
Language: Chinese, English version preview
Release Date: 2023
Published By: General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
Latest: GB 31604.55-2023

Introduction

1. Background and purpose of the standard

GB 31604.55-2023 aims to regulate the migration determination of isothiazolinone compounds in food contact materials and articles to ensure food safety. As people pay more attention to food packaging materials, the formulation of this standard fills the gap in the relevant field in China.

2. Overview of determination methods

This standard provides two determination methods: high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). These two methods are respectively applicable to different types of food simulants, ensuring the comprehensiveness and accuracy of the detection.

3. Principle and process of the method

Determination method	Main steps	Scope of application
High performance liquid chromatography (HPLC)	Direct injection after migration test Use ultraviolet detector or diode array detector Quantification by peak area external standard method	Water, ethanol-containing food simulants and chemical alternative solvents
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)	Select reaction monitoring mode Qualitative and quantitative analysis by multiple reaction monitoring (MRM) Optimization of mass spectrometry parameters such as nebulizer gas and spray voltage	Food simulants containing fats and oils and chemical alternative solvent isooctane

4. Experimental conditions and instrument requirements

High performance liquid chromatography (HPLC)

Chromatographic column: C₁₈ column that can tolerate high water phase, column length 150 mm
Mobile phase: A is water, B is methanol; elution gradient see standard documents
Detection wavelength: 275 nm, 1,2-benzisothiazolyl-3(2H)-one is 224 nm

Liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Ionization mode: electrospray ionization positive ion mode (ESI⁺)
Mass spectrometry scanning mode: multiple reaction monitoring (MRM)
Parameters such as nebulizer gas and spray voltage are shown in Appendix B of the standard document

5. Implementation suggestions

In actual operation, the appropriate method should be selected according to the sample type and intended use. For the migration test results, attention should be paid to the treatment of the blank test solution, and the specific migration amount should be calculated strictly according to the standard formula to ensure the accuracy and reliability of the data.

GB 31604.55-2023 history

2023 GB 31604.55-2023 National Food Safety Standard Determination of Migration Amount of Isothiazolinone Compounds in Food Contact Materials and Products

GB 31604.55-2023