GB 5009.36-2023

National Food Safety Standard Determination of Cyanide in Food (English Version)

Standard No.

GB 5009.36-2023

Language

Chinese, English version preview

Release Date

2023

Published By

General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China

Latest

GB 5009.36-2023

Replace By

GB 5009.36-2016

Introduction

GB 5009.36-2023 Standard Interpretation

Determination method	Scope of application	Detection principle	Main instrument	Sensitivity
Spectrophotometry	Distilled liquor and its blended liquor, edible alcohol, cassava flour, etc.	Use cyanide to react with isonicotinic acid-pyrazolone to generate a blue dye, and quantify it by absorbance measurement.	Visible spectrophotometer	Detection limit: 0.30 mg/kg (cassava flour), 0.03 mg/L (wine)
Gas chromatography	Liquor, edible alcohol, etc.	Separation of cyanide derivatives by gas chromatography, qualitative and quantitative analysis by electron capture detector.	Gas chromatograph (with ECD)	Detection limit: 0.02 mg/L (wine)
Gas chromatography-mass spectrometry	Wine and some beverages.	Combining gas chromatography and mass spectrometry technology, high-sensitivity qualitative and quantitative analysis is achieved.	Gas chromatograph-mass spectrometer (with ECD)	Detection limit: 0.02 mg/L (alcohol)
Ion chromatography	Packaged drinking water, mineral water and some beverages.	Use ion chromatography to separate cyanide, and amperometric detector to achieve high sensitivity detection.	Ion chromatograph (with PAD)	Detection limit: 0.0015 mg/L (drinking water)
Flow injection	Alcohol and beverages.	Online distillation combined with spectrophotometric quantification is suitable for large-scale sample testing.	Flow injection analyzer	Detection limit: 0.015 mg/L (alcohol)

Standard background and technology evolution analysis

Comparison between the old and new versions:

• Added the third method (gas chromatography-mass spectrometry) and the fifth method (flow injection/continuous flow-spectrophotometry).
• Deleted the third method qualitative method and optimized the reagent preparation process.
• Modified the scope of application and pretreatment steps of the standard.

Technical advantages:

• The detection sensitivity is significantly improved, which is suitable for the determination of trace cyanide.
• The methods are diversified to meet the detection needs of different sample types.
• Added mass spectrometry qualitative analysis to improve the accuracy of the results.

Implementation recommendations

1. Method selection:Choose an appropriate method based on the sample type, such as gas chromatography or flow injection for liquor, and ion chromatography for drinking water.
2. Laboratory conditions:Equipped with appropriate instruments and equipment, and ensure the traceability of reagents and standard substances.
3. Staff training:Focus on training in sample pretreatment, instrument operation and data processing skills.
4. Quality control:Perform method validation regularly to ensure the accuracy and reliability of test results.

Note: All testing operations must be completed in a fume hood. Cyanide is a highly toxic substance and laboratory safety regulations must be strictly observed.

GB 5009.36-2023 history

• 2023 GB 5009.36-2023 National Food Safety Standard Determination of Cyanide in Food
• 2017 GB 5009.36-2016 National food safety standard Determination of cyanide in food
• 2003 GB/T 5009.36-2003 Method for analysis of hygienic standard of grains
• 1996 GB/T 5009.36-1996 Method for analysis of hygienic standard of grain
• 1985 GB 5009.36-1985 Method for analysis of hygienic standard of grain

GB 5009.36-2023 -All Parts