GB 5009.140-2023

National Food Safety Standard Determination of Acesulfame Potassium in Foods (English Version)

Standard No.: GB 5009.140-2023
Language: Chinese, English version preview
Release Date: 2023
Published By: General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
Latest: GB 5009.140-2023
Replace By: GB/T 5009.140-2003

Introduction

GB 5009.140-2023 Standard Overview

This standard replaces GB/T 5009.140-2003 "Determination of acesulfame potassium in beverages" and is applicable to the determination of acesulfame potassium in milk and dairy products, frozen drinks, fruit products, vegetable products and other foods. The main changes include expanding the scope of application, optimizing sample pretreatment methods and reference conditions of detection instruments, and adding detection limit and quantification limit indicators.

Comparison of standard frameworks

Old standard (GB/T 5009.140-2003)	New standard (GB 5009.140-2023)
Applicable to the determination of acesulfame potassium in beverages	Expanded to a variety of food categories, including dairy products, fruit products, etc.
Unspecified detection limit and quantification limit	New detection limit (0.0006 g/kg) and quantification limit (0.002 g/kg)
Using a single detection method	Optimizing the sample pretreatment process and supporting a variety of instrument conditions

Requirements for preparation of reagents and materials

Key reagents:

Potassium ferrocyanide solution (92 g/L): weigh 106 g, dissolve in water and make up to 1000 mL.
Zinc acetate solution (183 g/L): weigh 219 g, dissolve in a small amount of water, add 32 mL of glacial acetic acid and make up to 1000 mL.
Ammonium sulfate solution (0.02 mol/L): weigh 2.64 g, dissolve in water and make up to 100 mL.

Preparation of standard substances:

Acesulfame potassium standard stock solution (1000 mg/L): weigh 100 mg of standard substance and make up to 100 mL.
Standard intermediate solution (100 mg/L): Pipette 10 mL of stock solution and dilute to 100 mL.
Standard series working solution: Prepare standard solutions with concentrations of 0.200, 1.00, 5.00, 10.0, 50.0 and 100 mg/L.

Instrument reference conditions

High performance liquid chromatography with UV detector or diode array detector, C₁₈ column (250 mm × 4.6 mm, 5 μm) is recommended. The mobile phase gradient elution program is shown in Table 1:

Time (min)	Phase A (%)	Phase B (%)
0	85	15
6	85	15
6.1	10	90
15	10	90
15.1	85	15
20	85	15

Sample processing and determination steps

Depending on the type of sample, potassium ferrocyanide/zinc acetate protein precipitation method or neutral alumina solid phase extraction column purification method is used for pretreatment. Liquid chromatography is used for quantitative detection, retention time is used for qualitative detection, and external standard method is used for quantitative detection.

Implementation suggestions

Laboratory precautions:

Ensure that the reagents are accurately prepared and the instruments are calibrated regularly.
Operators must undergo professional training and be familiar with liquid chromatographs and sample pretreatment procedures.
It is recommended to establish a record of the use of standard substances to ensure data traceability.

GB 5009.140-2023 history

2023 GB 5009.140-2023 National Food Safety Standard Determination of Acesulfame Potassium in Foods
2003 GB/T 5009.140-2003 Determination of acesulfame K in beverages
0000年 GB/T 16345-1996

GB 5009.140-2023 National Food Safety Standard Determination of Acesulfame Potassium in Foods was changed to GB/T 5009.140-2003 Determination of acesulfame K in beverages.

GB 5009.140-2023 -All Parts