GB 5009.129-2023

National Food Safety Standard Determination of Ethoxyquin in Foods (English Version)

Standard No.

GB 5009.129-2023

Language

Chinese, English version preview

Release Date

2023

Published By

General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China

Latest

GB 5009.129-2023

Replace By

GB/T 5009.129-2003

Introduction

National Standard of the People's Republic of China

National Food Safety Standard Determination of Ethoxyquin in Foods (GB 5009.129-2023)

Standard Development Background and Technology Evolution Analysis

This standard replaces the previous GB/T 5009.129-2003. The main changes include:

• Revised to “National Food Safety Standard Determination of Ethoxyquin in Foods”
• Liquid chromatography (LC) was added
• Sample treatment, chromatographic conditions and quantitative methods of gas chromatography (GC) were optimized

Comparative Analysis of Standard Frameworks

Items	Liquid Chromatography (LC)	Gas Chromatography (GC)
Principle	Extraction with n-hexane under alkaline conditions, reconstitution with acetonitrile containing ascorbic acid, and determination with fluorescence detector	Extraction with n-hexane under alkaline conditions, concentration with nitrogen blow-through, and determination with nitrogen-phosphorus detector
Reagents and Instruments	Liquid Chromatography with Fluorescence Detector, C18 Column	Gas Chromatography with Nitrogen-Phosphorus Detector, Weak Polarity Capillary Column
Standard Curve Range	4.00 ng/mL to 100 ng/mL	20.0 ng/mL to 400 ng/mL
Limit of detection and limit of quantification	Limit of detection: 0.01 mg/kg, limit of quantification: 0.02 mg/kg	Limit of detection: 0.01 mg/kg, limit of quantification: 0.02 mg/kg

Technical details and application cases

Liquid chromatography (LC)

Steps:

1. Sample preparation: Take 500 g of representative sample, add ascorbic acid and crush it.
2. Extraction: Extract with n-hexane, concentrate with nitrogen and then dissolve.
3. Chromatographic conditions: C₁₈ column, acetonitrile-water (65:35), flow rate 1 mL/min, fluorescence detector.

Gas chromatography (GC)

Steps:

1. Sample preparation: Same as LC method.
2. Extraction: n-hexane extraction, nitrogen concentration and reconstitution.
3. Chromatographic conditions: weak polar capillary column, nitrogen carrier gas, nitrogen phosphorus detector.

Implementation advice

Laboratory precautions:

• Strictly control the purity and storage conditions of reagents, especially standards.
• Instrument calibration: Regularly calibrate the fluorescence detector of the liquid chromatography and the nitrogen-phosphorus detector of the gas chromatography.
• Sample processing: Pay attention to the amount of ascorbic acid added and the storage conditions to avoid decomposition affecting the measurement.
• Data verification: Keep the standard curve, chromatogram and calculation records to ensure that the results meet the precision requirements (the difference between two measurements is ≤15%).

GB 5009.129-2023 history

• 2023 GB 5009.129-2023 National Food Safety Standard Determination of Ethoxyquin in Foods
• 2003 GB/T 5009.129-2003 Determination of ethoxyquin residues in fruits
• 1995 GB/T 15518-1995 Test method for ethoxyquin residues in fruits

GB 5009.129-2023 National Food Safety Standard Determination of Ethoxyquin in Foods was changed to GB/T 5009.129-2003 Determination of ethoxyquin residues in fruits.

GB 5009.129-2023 -All Parts