GB 5009.168-2016
National Food Safety Standards Determination of Fatty Acids in Food (English Version)
- Standard No.
- GB 5009.168-2016
- Language
- Chinese, Available in English version
- Release Date
- 2017
- Published By
- National Health Commission of the People's Republic of China
- Latest
- GB 5009.168-2016
- Replace
- GB/T 5009.168-2003 GB/T 17377-2008 GB/T 22223-2008 GB 5413.27-2010 GB/T 9695.2-2008 GB/T 17376-2008 SN/T 2922-2011 NY/T 91-1988
- Scope
- This standard specifies the method for determining the fatty acid content in food. This standard is applicable to the determination of total fat, saturated fat (acid), and unsaturated fat (acid) in food. The hydrolysis-extraction method in this standard is applicable to the determination of fatty acid content in food; the transesterification method is applicable to the determination of fatty acid content in oil samples with a free fatty acid content of no more than 2%.
- Introduction
Core Essence of Standard Specifications
This testing standard is applicable to 12 types of food, including infant formula milk powder, animal and plant oils and fats, etc. It clearly uses the hydrolysis-ether extraction method as the basic pretreatment method, and integrates the original 7 testing methods for the first time.
Detection method decision tree
Method selection Applicable scenarios Free fatty acid limit Method 1 Internal standard method All food categories General method Method 2 Transesterification method Vegetable oil/animal fat ≤2.0% Method 3 Acetyl chloride method Milk powder/anhydrous butter Moisture content <5% Key nodes of process control
- 100m poly(dicyandiamide) capillary column replaced the original glass column
- Double buffered step temperature program (100℃-230℃/four-stage temperature control)
- Precision control of Dow Corning pipette accuracy error ≤ 0.5μL
Standard Evolution Evaluation
Iteration highlights Major improvements Chromatographic column upgrade Separation efficiency increased by 34%/theoretical plate number reached 2000/m Detection efficiency optimization Maximum running time reduced to 82min Quality control rigor Quantitative limit increased to 0.0033g/100g High-risk reagent management specifications
15% boron trifluoride methanol must adopt an ice-salt water bath preparation system, and the acetyl chloride hydrolysis operation must be equipped with a chemical protective mask and splash guard. The pretreatment room should be equipped with a mechanical ventilation system with an air intake and exhaust rate of ＞30m³/min.
Matrix Application Guide
- pH gradient hydrolysis process is preferred for dairy samples
- For nut oils, refer to the FDA incremental month-on-month correction formula
- EPA/DHA projects must be verified by photodiode detection
GB 5009.168-2016 history
- 2017 GB 5009.168-2016 National Food Safety Standards Determination of Fatty Acids in Food
- 2010 GB 5413.27-2010 National food safety standard Determination of fatty acids in foods for infans and young children,milk and milk products
- 2008 GB/T 17376-2008 Animal and vegetable fats and oils.Preparation of methyl esters of fatty acids
- 2003 GB/T 5009.168-2003 Determination of eicosapentaenoic acid and docosahexaenoic acid in foods
- 1998 GB/T 17376-1998 Animal and vegetable fats and oils--Preparation of methyl esters of fatty acids
- 1997 GB/T 5413.4-1997 Milk powder and formula foods for infant and young children--Determination of linoleic acid content
- 1988 GB/T 9695.2-1988 Meat and meat products--Method for determination of fatty acids
GB 5009.168-2016 has been changed from GB/T 5009.168-2003 Determination of eicosapentaenoic acid and docosahexaenoic acid in foods.
GB 5009.168-2016 has been changed from GB/T 17377-2008 Animal and vegetable fats and oils.Analysis by gas chromatography of methyl esters of fatty acids.
GB 5009.168-2016 has been changed from GB/T 22223-2008 Determination of total fat,saturated fat, and unsaturated fat in foods.Hydrolytic extraction-gas chromatography.
GB 5009.168-2016 has been changed from GB 5413.27-2010 National food safety standard Determination of fatty acids in foods for infans and young children,milk and milk products.
GB 5009.168-2016 has been changed from GB/T 9695.2-2008 Meat and meat products.Determination of fatty acids.
GB 5009.168-2016 has been changed from GB/T 17376-2008 Animal and vegetable fats and oils.Preparation of methyl esters of fatty acids.
GB 5009.168-2016 has been changed from SN/T 2922-2011 Determination of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)in foods for export.Gas chromatography.
GB 5009.168-2016 has been changed from NY/T 91-1988 Determination of erucic acid in the oil of rapeseed.Gas chromatographic method.