GB 31610.1-2023
National Food Safety Standard: Inspection of Gnatostome in Animal-based Aquatic Products and Their Products (English Version)
- Standard No.
- GB 31610.1-2023
- Language
- Chinese, English version preview
- Release Date
- 2023
- Published By
- General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
- Latest
- GB 31610.1-2023
- Introduction
GB 31610.1-2023 Standard Overview
GB 31610.1-2023 "National Food Safety Standard for the Inspection of Gnatostoma larvae in Animal-derived Aquatic Products and Their Products" specifies the morphological and PCR inspection methods for Gnatostoma larvae in animal-derived aquatic products and their products. This standard is applicable to the detection of Gnatostoma larvae in muscle tissue of aquatic products such as freshwater fish and amphibians.
Main Detection Methods
Method Name Description Scope of Application Key Steps Morphological Method Identification is carried out by observing the morphological characteristics of Gnatostoma larvae under a microscope. Applicable to direct detection of live or fixed worms in samples. 1. Sample digestion and filtration; 2. Observation of worm morphology under a microscope; 3. Result determination. PCR method Use PCR technology to amplify the mitochondrial gene fragment of Gnatostoma nematodes and perform sequencing analysis. Applicable to further identification of suspected samples. 1. DNA extraction; 2. PCR amplification; 3. Electrophoresis detection; 4. Sequence alignment. Instruments and reagents
Biological microscope (100×~400×)Used to observe the morphological characteristics of worms.
PCR amplification instrument and gel imaging system are used for DNA amplification and detection.
electrophoresis instrument and high-speed centrifuge (≥12000 r/min) are used for sample processing and separation.
Implementation suggestions
In actual detection, it is recommended that:
- Strictly follow the standard operating procedures for sample processing and insect separation.
- For PCR methods, ensure the specificity and amplification efficiency of primer design.
- Regularly calibrate instruments and equipment to ensure the accuracy of test results.
GB 31610.1-2023 history
- 2023 GB 31610.1-2023 National Food Safety Standard: Inspection of Gnatostome in Animal-based Aquatic Products and Their Products