GB/T 22260-2023 (English Version)

Standard No.: GB/T 22260-2023
Language: Chinese, English version preview
Release Date: 2023
Published By: General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
Latest: GB/T 22260-2023
Replace By: GB/T 22260-2008

Scope: GB/T 22260-2023 Determination of protein anabolic hormones in feeds by liquid chromatography-tandem mass spectrometry. This standard replaces GB/T 22260-2008 Determination of methyltestosterone in feeds by high-performance liquid chromatography-tandem mass spectrometry. This document describes the methods for determination of methyltestosterone in feeds by high-performance liquid chromatography-tandem mass spectrometry. Liquid chromatography-tandem mass spectrometry method for determination of protein anabolic hormones. This document is applicable to testosterone, methyltestosterone, bodiron, mehandrosterone, androstenedione, dehydroepiandrosterone, androstenedione in compound feeds, concentrated feeds, concentrate supplements, additive premixed feeds and mixed feed additives. Determination of nandrolone propionate, stanozolol, melengestrol acetate, and progesterone. The detection limit of methyltestosterone, stanozolol and melengestrol acetate in this document is 0.005 mg/kg, and the detection limit of testosterone, bodiron, mestandrosterone, androstenedione, dehydroepiandrosterone, nandrolone, propylene glycol The detection limit of nandrolone acid and progesterone is 0.02 mg/kg; the quantification limit of methyltestosterone, stanozolol, and melengestrol acetate is 0.01 mg/kg, and the limit of quantification of testosterone, bodiron, mesterone, androstene The limit of quantification for diketone, dehydroepiandrosterone, nandrolone, nandrolone propionate, and progesterone is 0.05 mg/kg.

Introduction

1. Background and significance of the standard

GB/T 22260-2023 "Determination of anabolic hormones in feed by liquid chromatography-tandem mass spectrometry" is a standard method for detecting illegally added anabolic hormones in feed. This standard replaces the old version GB/T 22260-2008, and mainly makes important revisions to the detection limit, experimental procedures and data analysis methods.

2. Comparison of standard frameworks

Standard dimensions	Old version GB/T 22260-2008	New version GB/T 22260-2023
Detection limit	-	Newly added, the range is between 0.005 mg/kg and 0.05 mg/kg
Detection principle	Liquid chromatography-tandem mass spectrometry	Optimized experimental conditions and data analysis methods
Sample processing steps	7 steps, including extraction, purification, etc.	New vortex oscillation extraction method and optimized use of adsorbent

3. Professional interpretation of standard implementation

3.1 Detection principle and technical parameters

This standard uses liquid chromatography-tandem mass spectrometry (LC-TMS) as the core detection method. In the experiment, an electrospray ion source is used to achieve accurate identification of target compounds through multiple reaction monitoring mode (MRM).

3.2 Experimental step optimization

Extraction step:The vortex oscillation method is used instead of the traditional stirring method to improve sample processing efficiency.
Purification process:The use of PSA adsorbent is added to significantly reduce matrix interference.
Chromatographic conditions: The gradient elution program and column temperature settings were optimized to improve the separation effect.

GB/T 22260-2023 history

2023 GB/T 22260-2023 Determination of Anabolic Hormones in Feed by Liquid Chromatography-Tandem Mass Spectrometry
2008 GB/T 22260-2008 Determination of methyltestosterone in feeds-High performance liquid chromatography tandem mass spectrometry