GB/T 36840-2018 (English Version)

Standard No.: GB/T 36840-2018
Language: Chinese, Available in English version
Release Date: 2018
Published By: General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
Latest: GB/T 36840-2018

Scope: This standard specifies the quarantine and identification methods for maize wilt (Clavibacter michiganensis subsp. nebraskensis). This standard is applicable to the quarantine and identification of maize wilt in maize seeds and maize plant materials.

Introduction

Quarantine identification method for corn wilt - in-depth analysis of national standards

Test steps	Specific requirements	Technical specifications
Sample pretreatment	Pretreatment methods for seeds and plant materials	- Seed soaking: 200mL PBS buffer, 4°C overnight - Plant tissue sampling: 2mm×2mm at the junction of diseased and healthy tissues
PCR initial test	Rapid detection based on real-time fluorescence PCR	- Primer combination: PSA-7/PSA-R and 1184-F/1184-R - Amplification product length: 393bp and 390bp
Pathogen isolation	Colony purification based on culture medium	- Use sCNS and NA culture medium - Suspected colonies need to be purified 3 times

Explanation of professional terms and practical application cases

PCR detection: Modern molecular biology technology, used to quickly identify the presence of pathogens.

Biolog test: Confirm the characteristics of the strain through microbial metabolic profile analysis.

Actual case: CMN-positive samples were detected in imported corn seeds and need to be destroyed in accordance with the standard.

Background of standard formulation and technological evolution

This standard is based on internationally accepted plant quarantine regulations and is formulated in combination with my country's actual situation. In recent years, with the development of molecular biology technology, PCR detection methods have gradually replaced traditional morphological identification and become the main detection method.

Implementation suggestions

Strengthen laboratory personnel training to ensure that they master the latest detection technology.
Establish an information sharing mechanism between regions and promptly report epidemic dynamics.
Promote rapid detection equipment to improve port quarantine efficiency.

GB/T 36840-2018 history

2018 GB/T 36840-2018 Detection and identification of Clavibacter michiganensis subsp. nebraskensis