GB/T 36789-2018 (English Version)

Standard No.: GB/T 36789-2018
Language: Chinese, Available in English version
Release Date: 2018
Published By: General Administration of Quality Supervision, Inspection and Quarantine of the People‘s Republic of China
Latest: GB/T 36789-2018

Scope: Nested RT-PCR and fluorescent quantitative RT-PCR methods for detecting animal rabies virus nucleic acid Detection of viral nucleic acid in brain tissue, spinal cord, cerebrospinal fluid, salivary glands, saliva and rabies virus cell culture of animals suspected of rabies virus infection

Introduction

1. Background of standard formulation and analysis of technological evolution

Rabies is a highly contagious disease caused by members of the lyssavirus genus, and its diagnostic technology has always been the focus of global veterinary and public health fields. The formulation of GB/T 36789-2018 standard is based on the international standardization trend and the demand for technological progress, combined with the characteristics of rabies epidemic in my country, and established two detection methods: nested RT-PCR and fluorescent quantitative RT-PCR.

2. Interpretation of nucleic acid detection methods

Method name	Operation steps	Features and advantages
Nested RT-PCR	1. Sample processing and RNA extraction 2. Reverse transcription and cDNA synthesis 3. Outer PCR amplification 4. Inner PCR verification	High specificity:Confirm the target sequence through double amplification. High sensitivity:Suitable for early diagnosis and trace sample analysis.
Fluorescence quantitative RT-PCR	1. Sample processing and RNA extraction 2. Reverse transcription synthesis of cDNA 3. Fluorescence real-time amplification monitoring	High sensitivity:Real-time monitoring of Ct value. High degree of automation:Suitable for large-scale sample testing.

3. Implementation Recommendations

Laboratories should be equipped with the following resources when applying this standard: - Professional PCR amplifiers and fluorescence quantitative PCR instruments - Efficient biosafety measures - Adequate control samples and reagents and consumables

4. Comparison of domestic and international standard frameworks

Dimensions	Domestic standards (GB/T 36789-2018)	International standards (OIE recommendations)
Scope of application	Applicable to multiple types of samples such as brain tissue and spinal cord	Recommended for clinical sample diagnosis
Detection method	Nested RT-PCR and fluorescent quantitative RT-PCR	Real-time fluorescent RT-PCR is the mainstream
Technical indicators	Sensitivity:Ct value ≤32 is positive Specificity:No nonspecific amplification	Sensitivity:Ct value ≤30 is positive

GB/T 36789-2018 history

2018 GB/T 36789-2018 Nucleic acid detection of animal rabies virus