采用超高通量 4D timsTOF fleX MALDI-2 技术对高通量实验、反应监测和化学合成产物分析

High-throughput experimentation reaction monitoring and analysis of chemistry synthesis products with ultrahigh-throughput 4D timsTOF fleX MALDI-2 technology

MALDI mass spectrometry is uniquely suited for high-throughput experimentation (HTE) chemical reaction monitoring by coupling it with trapped ion mobility spectrometry (TIMS) on Bruker’s timsTOF fleX platform.

Abstract

MALDI-2, a novel ionization technique introduced recently on the timsTOF fleX, further increases accessible chemical space. TIMS provides the perfect complementary technology for HTE monitoring by MALDI by separating matrix ions and other interfering background using ion mobility. The combination of novel MALDI-2 and smartbeam 3D laser technology provides accurate, reproducible, and quantitative data for small molecules.

Confident identification and confirmation of chemical synthesis products is based on 4D physical properties including collisional cross-section, accurate mass, isotope pattern, and CID-MS/MS fragmentation. The timsTOF fleX MALDI-2 method requires low sample amounts (picomoles per analysis), offers high acquisition speed (typically in the range of 1 second/sample or less), and is well suited for automation.